casgi.blogg.se - Seamless

Terminal end homology is easily incorporated by PCR amplification with custom DNA oligos engineered using our free web tool.Ĭloning Efficiency, Flexibility, and Precision The kit employs a proprietary enzyme/buffer mix to assemble DNA fragments with shared terminal end homology without extra sequences or scars in the final construct (“seamless”).

GeneArt™ Seamless PLUS Cloning is a simple, two-step process, consisting of in vitro assembly followed by transformation into One Shot™ DH10B™ T1R SA competent E.

Diverse Applications-streamline many synthetic biology and molecular biology techniques through the rapid combination, addition, deletion, or exchange of DNA segmentsįor cloning of more than 4 DNA fragments or for final molecules larger than 110 Kb, please consider the GeneArt™ High-Order Genetic Assembly System.

Vector Flexibility-use our linear vector or a vector of your choice.

Free Tools-design your final construct and DNA oligos in silico using our free web-based tool that takes you step-by-step through your project.Precision and Efficiency-designed to let you clone what you want, where you want, in the orientation you want, and achieve up to 90% correct clones with no extra sequences left behind.Speed and Ease-clone up to 4 DNA fragments, with sequence of your choice, simultaneously in a single vector no restriction digestion, ligation, or recombination sites required.The improvements above are combined with these key benefits shared by all GeneArt™ Seamless Cloning and Assembly kits: Versatility: high capacity, broad-range conjugative vector that replicates in most Gram negative bacteria.Larger Constructs: create constructs up to 40 kb.

Increased Efficiency: pre-cloning option for large fragments for increased cloning efficiency.

However, Seamless PLUS offers several advantages over previous kits: Like our first generation Seamless Cloning and Assembly Kit, GeneArt™ Seamless PLUS Cloning and Assembly Kit is the complete kit for simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute or less, room temperature reaction.